Comparison of Direct Immunofluorescence and Giemsa Staining in Chlamydia trachomatis Follicular Conjunctivitis
نویسندگان
چکیده
منابع مشابه
بررسی قدرت تشخیصی روشهای ایمونوفلئورسانس مستقیم( (DFAو رنگ آمیزی گیمسا در مقایسه با روش PCR ژن Omp1 جهت شناسایی کلامیدیا تراکوماتیس در نمونه ملتحمه بیماران مبتلا به کونژنکتیویت فولیکولار مراجعه کننده به بیمارستان فارابی در سال 1392
Background and Aim: Chlamydia trachomatis is an obligate intracellular parasite responsible for ocular and genital infections in human. Chlamydia trachomatis is the most common cause of chronic follicular conjunctivitis and is also responsible for 20% of acute conjunctivitis cases. As a rapid diagnosis is important in the reducing the long-term squeal of the diseases, the objective of this stud...
متن کاملA comparison of the sensitivity of immunofluorescence and Giemsa for staining Chlamydia trachomatis inclusions in cycloheximide-treated McCoy cells.
In a clinical study of 190 men with non-gonococcal urethritis, Chlamydia trachomatis inclusions were sought in cycloheximide-treated McCoy cells by an indirect immunofluorescent antibody technique. The method was consistently reliable over a period of two years, and the results were obtained within 24 hours of a patient's attendance. The results correlated with those obtained by Giemsa staining...
متن کاملPrevalence of Chlamydia trachomatis pooled serotypes BDE and FGK in children with chronic follicular conjunctivitis.
PURPOSE To evaluate type-specific antichlamydial antibody IgG in children with chronic follicular conjunctivitis. METHODS A total of 90 serum samples from juvenile patients with chronic follicular conjunctivitis were collected in the Southeastern Anatolian region of Turkey where trachoma is still endemic. These samples were investigated regarding Chlamydia trachomatis pooled serotype-specific...
متن کاملIndirect immunofluorescence staining of Chlamydia trachomatis inclusions in microculture plates with monoclonal antibodies.
Indirect immunofluorescence (IF) staining, using a monoclonal antibody, detected two- to fourfold more inclusions than did iodine staining. Of 274 clinical specimens, 53 (19.3%) were positive by IF on passage 1 as compared with 33 (12%) by iodine staining (P less than 0.005). IF-stained inclusions in McCoy cells in the bottom of microculture wells were readily viewed with a long-focal-length ob...
متن کاملFollicular conjunctivitis caused by Chlamydia trachomatis in an infant Saharan population: molecular and clinical diagnosis.
AIMS To ascertain the prevalence of acute trachoma as a supposed endemic disease among infants in the Saharan refugee camps of Tindouf (Algeria) and to evaluate the efficacy of treatment with a single dose of azithromycin. METHODS 527 children aged between 3 and 17 were evaluated in the camp schools in October 2001. All the children were clinically and microbiologically examined, including sl...
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ژورنال
عنوان ژورنال: Zahedan Journal of Research in Medical Sciences
سال: 2015
ISSN: 2383-2894
DOI: 10.17795/zjrms-2181